Reference Materials for Cholesterol Assays

Preparation and characterization of candidate reference materials for telomerase assays.

BACKGROUND Telomerase has been measured in body fluids of cancer patients, and clinical tests for telomerase may have utility as noninvasive, cost-effective methods for the early detection of cancer. However, telomerase activity measured by common methods such as the telomerase repeat amplification protocol (TRAP) and telomerase reverse transcriptase catalytic subunit (hTERT) mRNA by reverse tr...

Ovine reference materials and assays for prion genetic testing

BACKGROUND Genetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests for scoring PRNP codons are essential tools for eradicating scrapie and for evaluating rare alleles for increased resistance to disease. In addition to those associated with scrapie, there are dozens more PRNP polymorphisms that ma...

Accuracy in cholesterol assays.

Commutability of two JCTLM-listed secondary reference materials for two commercial lithium assays.

Fig. 1. Regression analysis (regression line – continuous – and 95% prediction interval – dashed lines) to evaluate commutability of IRMM BCR-304 (triangle) and NIST SRM 956c (black circles) between direct potentiometry and colorimetry assays for serum lithium. Gray circles identify native serum samples. Commutability of two JCTLM-listed secondary reference materials for two commercial lithium ...

Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based Assays

The 2013-present Ebola virus outbreak in Western Africa has prompted the production of many diagnostic assays, mostly based on nucleic acid amplification technologies (NAT). The calibration and performance assessment of established assays and those under evaluation requires reference materials that can be used in parallel with the clinical sample to standardise or control for every step of the ...